赵进东 院士
蓝藻的基础与应用研究组
北京大学 生命科学学院 教授，博士生导师
电话：
传真：010-62754427
E-mail：jzhao@pku.edu.cn
1、蓝细菌光合作用及电子传递
2、蓝细菌异型胞分化与格式形成
3、利用蓝细菌进行合成生物学研究
发现特异降解光系统II D1蛋白的蛋白酶Alr3815。我们分离到的Alr3815是一种新的蛋白酶，其CysPc-结构域为依赖于Ca2+的半胱氨酸蛋白酶活性结构域，在营养细胞和异型胞内都仅有很少量的表达，是蓝细菌异型胞成熟过程中PSII的降解所必需的。
我们发现的Ca2+结合蛋白Ccbp是参与异型胞分化的重要蛋白。通过解析Ccbp在有无Ca2+结合条件下的三维结构，发现它有两个Ca2+结合位点。当其中一个强结合位点失活后，Ccbp即丧失调节异型胞分化的能力。我们的结果为研究Ca2+信号在异型胞分化过程中的作用机制提供了结构上的基础。
蓝细菌的藻胆体 (PBS) 中除连接蛋白CpcG1 (LRC) 外还存在另一种功能是未知的CpcG2-PBS。我们的研究发现在适合PBS 吸收的光照条件下，光能可以通过CpcG2-PBS直接传递到PSI，而这两种类型的PBS的能量传递过程是完全独立的。
我们利用EASI-MS技术对单细胞 (Synechocystis 6803, Synechococcus 7002) 和丝状体(Anabaena 7120) 蓝细菌不同生长阶段的脂类组成进行了快速、有效的研究，发现随着细菌的生长，三种蓝细菌的SQDG/PG比例会明显降低，并且证明PG比例的降低并不是磷元素缺乏造成的结果。同时同一种脂类的饱和度也会细菌的衰老发生剧烈变化。结合EASI-MS快速检测，我们获得了SQDG/PG比例和脂类饱和度在不同生长周期中变化的规律。这两个因素可以作为检测蓝细菌生长状态的指征。
通过分析藻胆体的结构，我们可以清楚地确定所有藻胆蛋白以及保守的连接蛋白结构域的位置。我们的证据表明位于藻胆体末端的两个关键蛋白：ApcE和ApcF在藻胆体与光系统II的相互作用中发挥了重要的作用。我们的结果为深入了解光能的吸收以及传递过程奠定了坚实的基础。
在Synechococcus sp. PCC 7002中大量生产分泌型I型纤维素。在Synechococcus sp. PCC 7002 细胞壁中存在天然的纤维素组分。我们将Synechococcus sp. PCC 7002作为生物反应器，将醋酸杆菌中的六个纤维素合成相关基因在cesA基因缺失突变体中过量表达，得到了高产的分泌型I型纤维素。我们的方法为利用光能与CO2进行生物合成以及生产生物燃料提供了新的思路。
Jia K, Yang MK, Liu X, Zhang Q, Cao GX, Ge F, Zhao JD. (2024) Deciphering the structure, function, and mechanism of lysine acetyltransferase cGNAT2 in cyanobacteria. Plant Physiol., 194: 634-661.
Lu Q, Liu YQ, Zhao JD, Yao M. (2024) Successive accumulation of biotic assemblages at a fine spatial scale along glacier-fed waters. iScience, 27: 109476.
Zhang SY, Yan Q, Zhao JD, Liu YQ, Yao M. (2024) Distinct multitrophic biodiversity composition and community organization in a freshwater lake and a hypersaline lake on the Tibetan Plateau. iScience, 27: 110124.
Chen XY, Li S, Zhao JD, Yao M. (2024) Passive eDNA sampling facilitates biodiversity monitoring and rare species detection. Environ. Int., 187: 108706.
Zheng LQ, Zhang ZD, Wang HR, Zheng ZG, Wang JY, Liu HY, Chen HL, Dong CX, Wang GP, Weng YX, Gao N, Zhao JD. (2023) Cryo-EM and femtosecond spectroscopic studies provide mechanistic insight into the energy transfer in CpcL-phycobilisomes.Nat. Commun.,14: 3961.
Yin L, Zheng ZG, Li YL, Li XY, Cheng D, Dong CX, Liu YX, Zhao JD. (2023) PatU3 plays a central role in coordinating cell division and differentiation in pattern formation of filamentous cyanobacterium Nostoc sp. PCC 7120. Sci. China-Life Sci., 66: 2896-2909.
Wang HR, Zheng ZG, Zheng LQ, Zhang ZD, Dong CX, Zhao JD.(2023) Mutagenic analysis of the bundle‑shaped phycobilisome from Gloeobacter violaceus. Photosynth. Res., 158: 81-90.
Lu Q, Cheng C, Xiao L, Li J, Li X, Zhao X, Lu Z, Zhao JD, Yao M. (2023) Food webs reveal coexistence mechanisms and community organization in carnivores. Curr. Biol., 33: 647-659.
Zhang S, Bi Y, Zhao JD, Yao M (2023) To the north: eDNA tracing of the seasonal and spatial dynamics of fish assemblages along the world’s largest water diversion project. J. Environ. Manage., 331: 117217.
Lu Q, Zhang SY, Du JQ, Liu Q, Dong CX, Zhao JD, Wang YF, Yao M (2023) Multi-group biodiversity distributions and drivers of metacommunity organization along a glacial–fluvial–limnic pathway on the Tibetan Plateau. Environ. Res., 220:115236.
Li XY, Huang CH, Wei PJ, Zhang K, Dong CX, Lan Q, Zheng ZG, Zhang ZD, Zhao JD. (2022) Attachment of ferredoxin: NADP+oxidoreductase to phycobilisomes is required for photoheterotrophic growth of the cyanobacterium Synechococcus sp. PCC 7002. Microorganisms, 10: 1313.
Zhang S, Zheng YT, Zhan AB, Dong CX, Zhao JD, Yao M. (2022) Environmental DNA captures native and non-native fish community variations across the lentic and lotic systems of a megacity. Sci. Adv., 8: eabk0097.
Zhang S, Lin MX, Liu JW, Chen JC, Liu D, Zhao JD, Yao M. (2022) A centenary tale: population genetic insights into the introduction history of the oriental fre-bellied toad (Bombina orientalis) in Beijing. BMC Ecol. Evol., 22: 117.
Chen XY, Kong YQ, Zhang S, Zhao JD, Li S, Yao M. (2022) Comparative evaluation of common materials as passive samplers of environmental DNA. Environ. Sci. Technol., 56: 10798-10807.
Yao M, Zhang S, Lu Q, Chen XY, Zhang SY, Kong YQ, Zhao JD. (2022) Fishing for fish environmental DNA: Ecological applications, methodological considerations, surveying designs, and ways forward. Mol. Ecol., 31: 5132-5164.
Zheng L, Zheng ZG, Li XY, Wang GP, Zhang K, Wei PJ, Zhao JD, Gao N. (2021) Structural insight into the mechanism of energy transfer in cyanobacterial phycobilisomes. Nat. Commun., 12: 5497.
Shao XN, Lu Q, Xiong MY, Bu HL, Shi XY, Wang DJ, Zhao JD, Li S, Yao M. (2021) Prey partitioning and livestock consumption in the world’s richest large carnivore assemblage. Curr. Biol., 31: 4887-4897.
Shao XN, Lu Q, Liu MZ, Xiong MY, Bu HL, Wang DJ, Liu SY, Zhao JD, Li S, Yao M. (2021) Generalist carnivores can be effective biodiversity samplers of terrestrial vertebrates. Front. Ecol. Environ., 9: 557-563.
Zheng YT, Zhang S, Lu Q, Zhang SY, Wang LJ, Hong ML, Nguyen T, Zhao JD, Yao M. (2021) Population genetic patterns of a mangrove-associated frog reveal its colonization history and habitat connectivity. Divers. Distrib., 27: 1584-1600.
Lu Q, Xiao LY, Cheng C, Lu Z, Zhao JD, Yao M. (2021) Snow leopard dietary preferences and livestock predation revealed by fecal DNA metabarcoding: no evidence for apparent competition between wild and domestic prey. Front. Ecol. Evol., 9: 783546.
Zhang S, Zhao JD, Yao M (2020) A comprehensive and comparative evaluation of primers for metabarcoding eDNA from fish. Methods Ecol. Evol., 11: 1609-1625.
Liu X, Yang MK, Liu YF, Ge F, Zhao JD (2020) Structural and functional insights into a lysine deacylase in the cyanobacterium Synechococcus sp. PCC 7002. Plant Physiol., 184: 762-776.
董春霞、郑正高、王宏蕊、韦佩君、李祎林、张雪昂、李歆睿、张天逸
