Yangming Wang, Ph.D.
Cellular and molecular engineering group
Professor, College of Future Technology, Peking University
tel：
E-mail：yangming.wang@pku.edu.cn
RNA biology and stem cell biology. Utilizing approaches from cell biology, molecular biology, genetics, biochemistry, and bioinformatics, combined with new technologies such as next-generation sequencing, high-throughput screening, and gene editing, the research focuses on the following areas: the mechanisms of stem cell-related biological processes, disease models and drug target research, and the development of new technologies related to basic or applied research.
His major contribution to the field includes: systematic dissection of miRNA functions in embryonic stem cells, identification of a long noncoding RNA Trincr1 that regulates the self-renewal of embryonic stem cells through inhibiting ERK signaling, invention of a miRNA-inducible CRISPR platform (MICR) that can serve as miRNA reporter and cell specific genome editing tools, invention of a CRISPR based reporter for monitoring promoter activity (GRIT), identification of SUMO2-PIAS4-DPPA2/4 pathway that regulates the totipotency transition of embryonic stem cells and zygotic genome activation, identification and development of a new mitochondrial base editing tool, development of a method to identify conserved long noncoding RNAs of vertebrates through collaboration, which revealed novel rules about the conservation of long noncoding RNAs. He is currently using multiple-disciplinary engineering approaches to develop novel tools for basic and translational research in RNA and stem cell field. He has published over 40 scientific journal papers on peer-reviewed journals including Nature Genetics, Nature Cell Biology and Cell Research etc. He is a council member of Chinese Society of Stem Cell Research and Chinese Society of RNA Biology.
Ding S, Xie G, Han Z, Wang YM, Shi M, Zhai F, Liu T, Xie Z, Zhang W, Wu Y, Yang X, Zhou A, Fang F, Ren S, Liang S, Cao H, Xiong H, Ding C, Dai L. (2024) The clinical spectrum and pathogenesis associated with KMT2B variants in Chinese pediatric patients. Parkinsonism & Related Disorders, 129: 107172.
Li Z, Li X, Lin J, Wang YM, Cao H, Zhou J. (2024) Reevaluation by the CRISPR/Cas9 knockout approach revealed that multiple pluripotency-associated lncRNAs are dispensable for pluripotency maintenance while Snora73a/b is essential for pluripotency exit. Sci. China-Life Sci., 67: 2198-2212.
Huang W, Xiong T, Zhao Y, Heng J, Han G, Wang P, Zhao Z, Shi M, Li J, Wang J, Wu Y, Liu F, Xi JJ, Wang YM, Zhang QC. (2024) Computational prediction and experimental validation identify functionally conserved lncRNAs from zebrafish to human. Nat. Genet., 56: 124-135.
Shi F, Zhang K, Cheng Q, Che S, Zhi S, Yu Z, Liu F, Duan F, Wang YM, Yang N. (2024) Molecular mechanism governing RNA-binding property of mammalian TRIM71 protein. Sci. Bull., 69: 72-81.
Wei X, Yu S, Zhang T, Liu L, Wang X, Wang X, Chan YS, Wang YM, Meng S, Chen YG. (2023) MicroRNA-200 Loaded Lipid Nanoparticles Promote Intestinal Epithelium Regeneration in Canonical MicroRNA-Deficient Mice. ACS Nano, 17: 22901-22915.
Zhang XS, Xie G, Ma H, Ding S, Wu YX, Fei Y, Cheng Q, Huang Y, Wang YM. (2023) Highly reproducible and cost-effective one-pot organoid differentiation using a novel platform based on PF-127 triggered spheroid assembly. Biofabrication, 15, 045014.
Huang R, Liu J, Chen X, Zhi Y, Ding S, Ming J, Li Y, Wang YM, Na J. (2023) A long non-coding RNA LncSync regulates mouse cardiomyocyte homeostasis and cardiac hypertrophy through coordination of miRNA actions. Protein & Cell, 14: 153-157.
Mi L, Shi M, Li Y.X, Xie G, Rao X, Wu D, Cheng A, Niu M, Xu F, Yu Y, Gao N, Wei W, Wang X, Wang YM. (2023) DddA homolog search and engineering expand sequence compatibility of mitochondrial base editing. Nat. Commun., 14: 874.
Wang SH, Hao J, Zhang C, Duan FF, Chiu YT, Shi M, Huang X, Yang J, Cao H, Wang YM. (2022) Klf17 promotes human naive pluripotency through repressing MAPK3 and ZIC2. Sci. China-Life Sci., 65: 1985-1997.
Guo W, Wang S, Zhang X, Shi M, Duan F, Hao J, Gu K, Quan L, Wu Y, Liang Z, Wang YM. (2021) Acidic pH transiently prevents the silencing of self-renewal and dampens microRNA function in embryonic stem cells. Sci. Bull., 66: 1319-1329.
Zhao YT, Wang YM. (2021) Monitoring the promoter activity of long noncoding RNAs and stem cell differentiation through knock-in of sgRNA flanked by tRNA in an intron. Cell Discov., 7: 45.
Hao J, Yang X, Zhang C, Zhang XT, Shi M, Wang SH, Mi L, Zhao YT, Cao H, Wang YM. (2020) Klf3 promotes the 8-cell-like transcriptional state in pluripotent stem cells. Cell Prolif., 53: e12914.
Guo CJ, Ma XK, Xing YH, Zheng CC, Xu YF, Shan L, Zhang J, Wang S, Wang YM, Carmichael GG, Yang L, Chen LL. (2020) Distinct processing of lncRNAs contributes to non-conserved functions in stem cells. Cell, 181: 621-636.
Wu K, Liu H, Wang Y, He J, Xu S, Chen Y, Kuang J, Liu J, Guo L, Li D, Shi R, Shen L, Wang YM, Zhang X, Wang J, Pei D, Chen J. (2020) SETDB1-mediated cell fate transition between 2C-like and pluripotent states. Cell Rep., 30: 25-36.
Huiqing Cao, Lufeng Hu, Wang Yao, Kangwei Wang, Jing Wang, Ming Shi, Qixuan Cheng, Gang Xie, Shuangjin Ding, Yuxuan Li, Jie Wang, Yawen Liu, Guosheng Gao, Jiazhen Wang, Xiangyu Zhang, Zonglin Han, Ziyin Yang, Qirui Huang, Zili Wan
