Wensheng Wei, Ph.D.
Laboratory of Genome Editing
Professor, School of Life Sciences at Peking University
tel：
E-mail：wswei@pku.edu.cn
1.Genome editing and high-throughput functional genomics
2.Molecular mechanisms of host response to microbial pathogenicity
3.Molecular mechanisms of cancer and other human diseases
Wei group is interested in studying the molecular mechanisms of human diseases, especially the host response to microbial pathogenicity. The combination of forward and reverse genetic means is employed, often in a high-throughput fashion, for the identification of host genes of interest. Efforts have also been put into the development and application of eukaryotic gene editing techniques, such as TALENs and CRISPR/Cas9 systems.
Development of high-throughput screening based on genome editing technology. This high-impact work has much broader application in the functional identification of genes involved in variety of biological pathways and mechanisms. Using this technique, we has also successfully identified and clarified the essential membrane receptor proteins for Hepatitis C viral infection.
Development of novel technology termed ULtiMATE for the fast and efficient assembly of TAL effectors for the application in genome editing.
Complete decoding of TALE RVDs. Complete information regarding the DNA recognition preference of all potential RVDs of TALE will potentially improve the application of TAL effectors in bioengineering and precision therapy.
Identification of theClostridium difficiletoxin B’s receptor. Toxin B (TcdB) is the key virulence factor forC. difficileinfections (CDIs), the most significant antibiotics-resistance associated disease worldwide. The searching of TcdB receptor has been lasted for over 30 years. This is the first identification of such receptor, which has profound importance for better understanding and novel therapeutics of CDIs.
Yi ZY, Zhao YX, Yi ZX, Zhang YJ, Tang GB, Zhang XX, Tang HX, Zhang W, Zhao Y, Xu HY, Nie YY, Sun XQ, Xing LJ, Dai L, Yuan PF, Wei WS. (2023) Utilizing AAV-mediated LEAPER 2.0 for programmable RNA editing in non-human primates and nonsense mutation correction in humanized Hurler syndrome mice. Genome Biol., 24: 243.
Bao Y, Pan Q, Xu, Liu ZH, Zhang ZX, Liu YS, Xu YY, Yu Y, Zhou Z, Wei WS. (2023) Unbiased interrogation of functional lysine residues in human proteome. Mol. Cell, 83: 4614-4632.
Ma KY, Wang X, Wu LJ, Yu LL, Ye JH, Li XL, Geng LL, Shi ZY, Yang HH, Zhang XJ, Zhang YJ, Wu SC, Yuan PF, Zhang YC, Dong F, Hao S, Hu LP, Wei WS, Fang RG, Cheng T. (2023) CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment. iScience, 26: 108561.
Mi L, Shi M, Li Y.X., Xie G, Rao X, Wu D, Cheng A, Niu M, Xu F, Yu Y, Gao N, Wei WS, Wang X, Wang Y. (2023) DddA homolog search and engineering expand sequence compatibility of mitochondrial base editing. Nat. Commun. 14: 874.
Yi ZY, Qu L, Tang HX, Liu ZH, Liu Y, Tian F, Wang CH, Zhang XX, Feng ZQ, Yu Y, Yuan PF, Yi ZX, Zhao YX, Wei WS. (2022) Engineered circular ADAR-recruiting RNAs increase the efficiency and fidelity of RNA editing in vitro and in vivo. Nat. Biotechnol., 40: 946-955.
Lu SY, Yang CY, Tang C, Yang Y, Yu WH, Wang JB, Zhou YN, Huang Q, Yisimayi AYJ, Liu S, Huang WJ, Cao YL, Wang YC, Zhou Z, Peng XZ, Wang JW, Xie XL, Wei WS. (2022) Circular RNA vaccines against SARS-CoV-2 and emerging variants. Cell, 185: 1728-1744.
Liu Y, Ding B, Zheng LN, Xu P, Liu ZH, Chen Z, Wu PY, Zhao Y, Pan Q, Guo Y, Wang W, Wei WS. (2022) Regulatory elements can be essential for maintaining broad chromatin organization and cell viability. Nucl. Acids Res., 50: 4340-4354.
Wei WS, Gao CX. (2022) Gene editing: from technologies to applications in research and beyond. Sci. China-Life Sci., 65: 657-659.
Li GL, Li XY, Zhuang SK, Wang LR, Zhu YF, Chen YC, Sun W, Wu ZG Zhou Z, Chen J, Huang XX, Wang J, Li DL, Li W Wang HY, Wei WS. (2022) Gene editing and its applications in biomedicine. Sci. China-Life Sci., 65: 660-700.
Guo SJ, Chen YO, Liu JZ, Zhang XY, Liu ZH, Zhou Z, Wei WS. (2022) Low-density lipoprotein receptor-related protein 1 is a CROPs-associated receptor for Clostridioides difficile toxin B. Sci. China-Life Sci., 65: 107-118.
Zhu SY, Liu Y, Zhou Z, Zhang ZY, Xiao X, Liu ZH, Chen A, Dong XJ, Tian F, Chen SH, Xu YY, Wang CH, Li QH, Niu XR, Pan Q, Du S, Xiao JY, Wang JW,Wei WS. (2022) Genome-wide CRISPR activation screen identifies candidate receptors for SARS-CoV-2 entry.Sci. China-Life Sci., 65: 701-717.
Xu P Liu ZH, Liu Y, Ma HZ, Xu YY, Bao Y, Zhu SY, Cao ZZ, Wu ZG, Zhou Z,Wei WS. (2021) Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs.Nat. Biotechnol., 39: 1403-1413.
Zhou Z, Zhang XY, Lei XB, Xiao X, Jiao T, Ma RY, Dong XJ, Jiang Q, Wang WJ, Shi YJ, Zheng T, Rao J, Xiang ZC, Ren LL, Deng T, Jiang ZF, Dou ZX,Wei WS, Wang JW. (2021) Sensing of cytoplasmic chromatin by cGAS activates innate immune response in SARS-CoV-2 infection.Signal Transduct. Target. Ther., 6: 382.
Ding B, Liu Y, Liu ZH, Zheng LN, Xu P, Chen Z, Wu PY, Zhao Y, Pan Q, Guo Y,Wei WS, Wang W. (2021) Noncoding loci without epigenomic signals can be essential for maintaining global chromatin organization and cell viability.Sci. Adv., 7: eabi6020.
Liang YS, Zhang GG, Li QH, Han L, Hu XY, Guo Y, Tao WY, Zhao XM, Guo MZ, Gan TY, Tong YM, Xu YF, Zhou Z, Ding Q,Wei WS, Zhong J. (2021) TRIM26 is a critical host factor for HCV replication and contributes to host tropism.Sci. Adv., 7: eabd9732.
Liu Y, Liu ZH, Cao ZZ,Wei WS. (2020) Reply to: Fitness effects of CRISPR/Cas9-targeting of long noncoding RNA genes.Nat. Biotechnol.,38: 577-578.
牛煦然，周卓，魏文胜. (2020) 浅谈2020年诺贝尔化学奖：通向未来的基因编辑。大学化学，35: 118–126.
Zhao X, Zhang GG, Liu S, Chen XP, Peng RC, Dai LP, Qu X, Li SH, Song H, Gao ZR, Yuan PF, Liu ZH, Li CY, Shang ZF, Li Y, Zhang MF, Qi JX, Wang H, Du N, Wu Y, Bi YH, Gao S, Shi Y, Yan JH, Zhang Y, Xie ZD,Wei WS, Gao GF. (2019) Human neonatal Fc receptor is the cellular uncoating receptor for enterovirus B.Cell, 177: 1553-1565.
Qu L, Yi ZY, Zhu SY, Wang CH, Cao ZZ, Zhou Z, Yuan PF, Yu Y, Tian F, Liu ZH, Bao Y, Zhao YX,Wei WS. (2019) Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs.Nat. Biotechnol., 37: 1059-1069.
Zhang GG, Zhou Z,Wei WS. (2019) In vivo ways to unveil off-targets.Cell Res., 29: 339-340.
Zhu SY, Cao ZZ, Liu ZH, He Y, Wang YA, Yuan PF, Li W, Tian F, Bao Y,Wei WS. (2019) Guide RNAs with embedded barcodes boost CRISPR-pooled screens.Genome Biol., 20: 20.
Zhou Z,Wei WS. (2019) Interrogating the noncoding genome in a high-throughput fashion.Natl. Sci. Rev., 6: 397-399.
Lander ES, Baylis F, Zhang F, Charpentier E, Berg P, Bourgain C, Friedrich B, Joung JK, Li JS, Liu D, Naldini L, Nie JB, Qiu RZ, Schoene-Seifert B, Shao F, Terry S,Wei WS, Winnacker EL. (2019) Adopt a moratorium on heritable genome editing.Nature, 567: 165-168.
Zhang XY, Yue D, Wang YN, Zhou YX, Liu Y, Qiu YT, Tian F, Yu Y, Zhou Z, andWei WS. (2019) PASTMUS: mapping functional elements at single amino acid resolution in human cells. Genome Biol.,20: 279.
Zeguang Wu, Yin Liu, Yuting Chen, Yin Liu, Bei Wang, Yin Yu, Lei Dong, Xue Zhang, Ming Ge, Yongshuo Liu, Zongyi Yi, Deli Song, Chunhui Wang, Ying Bao, Xiaoxue Zhang, Yizhou Li, Qian Pan, Feng Tian, Lamaoqiezhong, Ang Chen, Yiyuan Xu, Wenjuan Zheng Xuran Niu, Huixian Tang, Wei Zhang, Yong Sheng, Yuanyuan Qiu, Yuxuan Liu, Feng Chen, Xuanxuan Ji, Jinxin Yang, Wei Tang, Lei Xi, Zhixuan Zhang, Yangfang Xiong, Ziying Yan, Gexing Liu, Xiaoxu Wei, Rong Yang, Zixu Gao, Xiwen Chang, Yanglong Sun, Jiwu Ren, Aojie Zhang, Hongbo Su, Xinpeng Chen, Bohan Li, Jiayi Li, Lu Wang, Binrui Mo
