汪阳明 教授
细胞与分子工程研究组
北京大学 未来技术学院 教授，博士生导师
电话：
E-mail：yangming.wang@pku.edu.cn
研究领域主要为干细胞、RNA生物学及基因编辑。利用细胞生物学、分子生物学、遗传学、生物化学和生物信息学等手段，结合二代测序、高通量筛选、基因编辑等新技术进行研究，研究内容涵盖干细胞相关生物学过程机制研究，疾病模型和药物靶标研究，以及与基础或应用研究相关的共性新技术开发。
主要成果包括建立了miRNA全敲除小鼠胚胎干细胞系，率先揭示了miRNA通路对于胚胎干细胞增殖与分化的重要作用；发现了调控胚胎干细胞增殖、细胞周期、糖酵解代谢和自我更新等功能的miR-290和miR-302家族并解析了其分子机制；发明了miRNA激活CRISPR-Cas9基因编辑体系开启的技术平台，以及新型非编码RNA启动子活性报告基因技术；鉴定了调控FGF/ERK通路活性的长非编码RNA，解析了其促进胚胎干细胞自我更新的功能和分子机制；发现了调控早期胚胎发育合子基因组激活 （ZGA） 和体外全能干细胞转化的新分子通路PIAS4-SUMO2-DPPA2/4；发展了新型线粒体碱基编辑器和新型类器官培养平台；合作发展了鉴定非编码RNA保守性的新方法，揭示了非编码RNA保守性新规律。在学术杂志Nature Genetics、Nature Cell Biology、Nature Communications、EMBO Journal、Cell Research和PLoS Biology等以第一或通讯作者发表学术论文40余篇，总引用6000余次。现任Cell Proliferation编委，中国细胞生物学学会干细胞分会委员，中国生物化学及分子生物学学会RNA分会委员，RNA分会青年委员会主任委员。
Ding S, Xie G, Han Z, Wang YM, Shi M, Zhai F, Liu T, Xie Z, Zhang W, Wu Y, Yang X, Zhou A, Fang F, Ren S, Liang S, Cao H, Xiong H, Ding C, Dai L. (2024) The clinical spectrum and pathogenesis associated with KMT2B variants in Chinese pediatric patients. Parkinsonism & Related Disorders, 129: 107172.
Li Z, Li X, Lin J, Wang YM, Cao H, Zhou J. (2024) Reevaluation by the CRISPR/Cas9 knockout approach revealed that multiple pluripotency-associated lncRNAs are dispensable for pluripotency maintenance while Snora73a/b is essential for pluripotency exit. Sci. China-Life Sci., 67: 2198-2212.
Huang W, Xiong T, Zhao Y, Heng J, Han G, Wang P, Zhao Z, Shi M, Li J, Wang J, Wu Y, Liu F, Xi JJ, Wang YM, Zhang QC. (2024) Computational prediction and experimental validation identify functionally conserved lncRNAs from zebrafish to human. Nat. Genet., 56: 124-135.
Shi F, Zhang K, Cheng Q, Che S, Zhi S, Yu Z, Liu F, Duan F, Wang YM, Yang N. (2024) Molecular mechanism governing RNA-binding property of mammalian TRIM71 protein. Sci. Bull., 69: 72-81.
Wei X, Yu S, Zhang T, Liu L, Wang X, Wang X, Chan YS, Wang YM, Meng S, Chen YG. (2023) MicroRNA-200 Loaded Lipid Nanoparticles Promote Intestinal Epithelium Regeneration in Canonical MicroRNA-Deficient Mice. ACS Nano, 17: 22901-22915.
Zhang XS, Xie G, Ma H, Ding S, Wu YX, Fei Y, Cheng Q, Huang Y, Wang YM. (2023) Highly reproducible and cost-effective one-pot organoid differentiation using a novel platform based on PF-127 triggered spheroid assembly. Biofabrication, 15, 045014.
Huang R, Liu J, Chen X, Zhi Y, Ding S, Ming J, Li Y, Wang YM, Na J. (2023) A long non-coding RNA LncSync regulates mouse cardiomyocyte homeostasis and cardiac hypertrophy through coordination of miRNA actions. Protein & Cell, 14: 153-157.
Mi L, Shi M, Li Y.X, Xie G, Rao X, Wu D, Cheng A, Niu M, Xu F, Yu Y, Gao N, Wei W, Wang X, Wang YM. (2023) DddA homolog search and engineering expand sequence compatibility of mitochondrial base editing. Nat. Commun., 14: 874.
Wang SH, Hao J, Zhang C, Duan FF, Chiu YT, Shi M, Huang X, Yang J, Cao H, Wang YM. (2022) Klf17 promotes human naive pluripotency through repressing MAPK3 and ZIC2. Sci. China-Life Sci., 65: 1985-1997.
Guo W, Wang S, Zhang X, Shi M, Duan F, Hao J, Gu K, Quan L, Wu Y, Liang Z, Wang YM. (2021) Acidic pH transiently prevents the silencing of self-renewal and dampens microRNA function in embryonic stem cells. Sci. Bull., 66: 1319-1329.
Zhao YT, Wang YM. (2021) Monitoring the promoter activity of long noncoding RNAs and stem cell differentiation through knock-in of sgRNA flanked by tRNA in an intron. Cell Discov., 7: 45.
Hao J, Yang X, Zhang C, Zhang XT, Shi M, Wang SH, Mi L, Zhao YT, Cao H, Wang YM. (2020) Klf3 promotes the 8-cell-like transcriptional state in pluripotent stem cells. Cell Prolif., 53: e12914.
Guo CJ, Ma XK, Xing YH, Zheng CC, Xu YF, Shan L, Zhang J, Wang S, Wang YM, Carmichael GG, Yang L, Chen LL. (2020) Distinct processing of lncRNAs contributes to non-conserved functions in stem cells. Cell, 181: 621-636.
Wu K, Liu H, Wang Y, He J, Xu S, Chen Y, Kuang J, Liu J, Guo L, Li D, Shi R, Shen L, Wang YM, Zhang X, Wang J, Pei D, Chen J. (2020) SETDB1-mediated cell fate transition between 2C-like and pluripotent states. Cell Rep., 30: 25-36.
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