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Assistant Professor Nam Ki LeeSeminal title: Single-molecule fluorescence spectroscopy for biological systems

Nam Ki Lee  Assistant Professor

School of Interdisciplinary Bioscience and Bioengineering and Department of Physics, POSTECH, Korea

Seminal title: Single-molecule fluorescence spectroscopy for biological systems

Place and location: Room 517, Biotechnology Building, 2010-4-14, 2:00PM

   Abstract 
    Recent advances in single-molecule fluorescence microscope techniques have allowed probing various biological reactions, such as protein-DNA interactions, conformational change of proteins and protein-DNA complexes, and basic cellular processes occurring in cells. Fluorescence resonance energy transfer (FRET) has been widely used for such single-molecule studies. Here, I will present a novel single-molecule technique, named alternating-laser excitation fluorescence resonance energy transfer (ALEX-FRET) of single molecules. This method can measure up to three intramolecular distances in nanometer scales and complex interactions of biomolecules in solution. Using triply labeled DNA, we proved that ALEX enables FRET-independent analysis of up to three-component interactions, observation and sorting of singly, doubly, and triply labeled molecules, and measurements of three intramolecular distances within single molecules from a single measurement. Then we applied ALEX to study the translocation of RNA polymerase on DNA, the folding of nanostructured DNA (8-17 DNAzyme), oligomerization of recombination protein (RecR), and vesicle fusion by SNARE proteins. These results show clear advantages of ALEX and its future applications for further biological studies.
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