Jianguo Ji, Professor
Proteomics research
Professor, College of Life Sciences, Peking University
tel：
E-mail：jijg@pku.edu.cn
1.Protein high throughput and high confident identification, quantification and modification characterization by mass spectrometry
2.Protein changes in neurodegenerative diseases and microglia activation
3.Neoantigens in the surface of tumour cell and tumour infiltrating lymphocytes and their regulation mechanism in tumour microenvironment.
4.Cell-free preparation of the specific antibody and ADC based on the tumour neoantigen targets.
Protein expression and modifications play key roles in regulating the cell’s function and human disease state. We mainly focuses on the protein expression changes in brain aging, neurodegenerative diseases, cancer cell surface protein targets and biomarkers by quantitative proteomics. Ongoing projects include protein quantification, acetylation and phosphorylation characterization in AD and PD, high throughput profiling of CRC cancer cell surface specific protein targets and development of the new antibody-drug conjugates.
Yao PJ, Gao MJ, Hu WY, Wang JH, Wang YH, Wang QS, Ji JG. (2024) Proteogenomic analysis identifies neoantigens and bacterial peptides as immunotherapy targets in colorectal cancer. Pharmacol. Res., 204: 107209.
Che R, Wang QS, Li MZ, Shen J, Ji JG. (2024) Quantitative proteomics of tissue-infiltrating T cells from CRC patients identified lipocalin-2 induces T cell apoptosis and promotes tumor cell proliferation by iron efflux. Mol. Cell. Proteomics, 23:100691.
Zhu WY, Li MZ, Wang QS, Shen J, Ji JG. (2024) Quantitative proteomic analysis reveals functional alterations of the peripheral immune system in colorectal cancer. Mol. Cell Proteomics, 23: 100784.
Zhang WY, Sun SH, Zhu WY, Meng DL, Hu WY, Yang SQ, Gao MJ, Yao PJ, Wang YH, Wang QS, Ji JG. (2024) Birinapant reshapes the tumor immunopeptidome and enhances antigen presentation. Int. J. Mol. Sci., 25: 3660.
Shen J, Lai WJ, Li ZY, Zhu WY, Bai X, Yang ZH, Wang QS, Ji JG. (2024) SDS3 regulates microglial inflammation by modulating the expression of the upstream kinase ASK1 in the p38 MAPK signaling pathway. Inflamm. Res., 73: 1547-1564.
Shen J, Zhao XY, Bai X, Zhu WY, Li ZY, Yang ZH, Wang QS, Ji JG. (2024) Phosphoproteomic analysis reveals CDK5-Mediated phosphorylation of MTDH inhibits protein synthesis in microglia. Biochem. Biophys. Res. Commun., 735: 150669.
Lin YT, Jiang HG, Li J, Ren FL, Wang YY, Qiu Y, Li JH, Li MD, Wang Y, Yang L, Song YH, Jia HH, Zhai WL, Kuang YS, Yu HY, Zhu WY, Liu SL, Morii E, Ensinger C, David C, Zheng HQ, Ji JG, Wang HX, Chang ZJ. (2024) Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization. Theranostics, 14: 662-680.
Zhu WY, Yang SQ, Meng DL, Wang QS, Ji JG. (2023) Targeting NADPH oxidase and integrin α5β1to inhibit neutrophil extracellular traps-mediated metastasis in colorectal cancer. Int. J. Mol. Sci., 24: 16001.
Zeng M, Tang ZZ, Ren LFD, Wang HB, Wang XJ, Zhu WY, Mao XB, Li ZY, Mo XM, Chen J, Han JH, Kong DC,Ji JG, Carr AM, Liu C. (2023) Hepatitis B virus infection disrupts homologous recombination in hepatocellular carcinoma by stabilizing resection inhibitor ADRM1.J. Clin. Invest., 133: e171533.
Yang NF, Wang YX, Tian Q, Wang QP, Lu Y, Sun YC, Wang SJ, Bei YC, Ji JG, Zhou H, Yang W, Yao PJ, Zhu WY, Sun LY, Huang ZF, Li XK, Shen PP. (2023) Blockage of PPARγ T166 phosphorylation enhances the inducibility of beige adipocytes and improves metabolic dysfunctions. Cell Death Differ., 30: 766-778.
Li SP, Li XY, Yang SQ, Pi H, Li ZY, Yao PJ, Zhang Q, Wang QS, Shen PP, Li XZ, Ji JG. (2021) Proteomic landscape of exosomes reveals the functional contributions of cd151 in triple-negative breast cancer. Mol. Cell. Proteomics., 20: 100121.
Liu SJ, Hua Y, Wang JN, Li LY, Yuan JJ, Zhang B, Wang ZY, Ji JG, Kong DC. (2021) RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination. Cell, 184: 1314-1329.
Liu Y, Wang L, Xu X, Yuan Y, Zhang B, Li ZY, Xie YC, Yan R, Zheng ZQ, Ji JG, Murray JM, Carr AM, Kong DC. (2021) The intra-S phase checkpoint directly regulates replication elongation to preserve the integrity of stalled replisomes. Proc. Natl. Acad. Sci. U. S. A., 118: e2019183118.
Xu BY, Zhu L, Wang QS, Zhao YF, Jia M, Shi SF, Lui LJ, Lv JH, Lai WJ, Ji JG, Zhang H. (2021) Mass spectrometry-based screening identifies circulating immunoglobulinA–α1-microglobulin complex as potential biomarker in immunoglobulin A nephropathy. Nephrol. Dial. Transplantat., 36: 782-792.
Bei YC, Cheng N, Chen T, Shu YX, Yang Y, Yang NF, Zhou XY, Liu BR, Wei J, Liu Q, Zheng W, Zhang WL, Su HF, Zhu WG, Ji JG, Shen PP. (2020) CDK5 inhibition abrogates TNBC stem-cell property and enhances Anti-PD-1 therapy. Adv. Sci., 202001417.
Qingsong Wang, Wenyuan Zhu, Yuanpeng Tong, Lei Wang, Weiyan Zhang, Delan Meng, Weiyi Hu, Mingjie Gao, Siqi Yang, Yuhao Wang
